1) The plasmid (3500 bp in length) is digested with EcoRI and XbaI. We assume th

1) The plasmid (3500 bp in length) is digested with EcoRI and XbaI. We assume that the
loss of nucleotides located between the two restriction sites is negligible.
a) A fragment of DNA (F 500 bp in length) was obtained by digestion of genomic DNA
with the restriction enzymes EcoRI and SpeI.The cleaved plasmid and cleaved fragment F are mixed together and then treated with
DNA ligase.
Bacteria unable to grow in the presence of kanamycin and ampicillin were transformed
with the ligation mixture.Transformed bacteria are plated either on dishes containing ampicillin or on dishes
containing kanamycin. Do you expect any colonies on the plates? Explain(4 pts)