How would you clone (isolate) the receptor cDNA or gene which mediates the contr

How would you clone (isolate) the receptor cDNA or gene
which mediates the contraction of heart tissue? Provide at least twoapproaches.Once you isolate the cDNA for the receptor involved in
mediating heart contraction you sub-clone the receptor cDNA into an expression
plasmids and then stably transfect COS cells and select the receptor-expressing
COS-45 cell line. Using your receptor-expressing COS-45
cells how would you demonstrate experimentally that the selective
antagonist binds thereceptorspecifically?
Describe the experiment you would perform and also the controls you would
include.
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